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	<title>Comments on: Eat food, not too much, mostly plants</title>
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	<link>http://www.biofortified.org/2009/08/eat-food-not-too-much-mostly-plants/</link>
	<description>Stronger plants, stronger science, and stronger communication.</description>
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		<title>By: Dennis Lusk</title>
		<link>http://www.biofortified.org/2009/08/eat-food-not-too-much-mostly-plants/#comment-2333</link>
		<dc:creator>Dennis Lusk</dc:creator>
		<pubDate>Sat, 26 Sep 2009 08:01:57 +0000</pubDate>
		<guid isPermaLink="false">http://sandbox.geneticmaize.com/?p=170#comment-2333</guid>
		<description>&lt;p&gt;Like I said, let&#039;s do the science, lets get a technology to work before subsidizing it on an industrial scale with borrowed money. What is wrong with seeing what works efficiently with less waste before committing taxpayers money, far into the future, on a project that, at best, creates starvation in the third world? I would think that some sort of modified cyanobacteria would be the best at collecting and storing sunlight as sugar, which is what all plants do. I would think, because of the danger, that a death cycle would have to be an integral part of that one.&lt;/p&gt;&lt;p&gt;&lt;br/&gt;Like government healthcare, besides being anti-Constitutional, lets see it work before jumping off an unknown cliff, like the proverbial lemming, and committing trillions to another astronomical government boondoggle that you will be paying many times over for the rest of your life. Let&#039;s do the due diligence and see what works.&lt;/p&gt;</description>
		<content:encoded><![CDATA[<p>Like I said, let&#8217;s do the science, lets get a technology to work before subsidizing it on an industrial scale with borrowed money. What is wrong with seeing what works efficiently with less waste before committing taxpayers money, far into the future, on a project that, at best, creates starvation in the third world? I would think that some sort of modified cyanobacteria would be the best at collecting and storing sunlight as sugar, which is what all plants do. I would think, because of the danger, that a death cycle would have to be an integral part of that one.</p>
<p>Like government healthcare, besides being anti-Constitutional, lets see it work before jumping off an unknown cliff, like the proverbial lemming, and committing trillions to another astronomical government boondoggle that you will be paying many times over for the rest of your life. Let&#8217;s do the due diligence and see what works.</p>
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		<title>By: Anastasia</title>
		<link>http://www.biofortified.org/2009/08/eat-food-not-too-much-mostly-plants/#comment-2332</link>
		<dc:creator>Anastasia</dc:creator>
		<pubDate>Fri, 25 Sep 2009 11:56:40 +0000</pubDate>
		<guid isPermaLink="false">http://sandbox.geneticmaize.com/?p=170#comment-2332</guid>
		<description>&lt;p&gt;That is true of grain ethanol, Dad, but there are other biofuels that aren&#039;t so harmful - particularly biofuels from perennial grasses and from algae. Biodiesel from soy and other oil sources are also promising. More research needs to be done of course. Here at Iowa State, we have a newly opened &quot;New Century Farm&quot; dedicated to exploring some of these possibilities, including their impacts on soil and water. It&#039;s so new, they barely have a &lt;a href=&quot;http://www.extension.iastate.edu/Bioeconomy/NewCenturyFarm&quot; rel=&quot;nofollow&quot;&gt;website&lt;/a&gt; up. They&#039;ll also have projects on processing the feedstocks to make fuel production more efficient. I&#039;ve seen it myself and I&#039;m really looking forward to seeing the research that comes out of the plant.&lt;/p&gt;</description>
		<content:encoded><![CDATA[<p>That is true of grain ethanol, Dad, but there are other biofuels that aren&#8217;t so harmful &#8211; particularly biofuels from perennial grasses and from algae. Biodiesel from soy and other oil sources are also promising. More research needs to be done of course. Here at Iowa State, we have a newly opened &quot;New Century Farm&quot; dedicated to exploring some of these possibilities, including their impacts on soil and water. It&#8217;s so new, they barely have a <a href="http://www.extension.iastate.edu/Bioeconomy/NewCenturyFarm" rel="nofollow">website</a> up. They&#8217;ll also have projects on processing the feedstocks to make fuel production more efficient. I&#8217;ve seen it myself and I&#8217;m really looking forward to seeing the research that comes out of the plant.</p>
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		<title>By: Dennis Lusk</title>
		<link>http://www.biofortified.org/2009/08/eat-food-not-too-much-mostly-plants/#comment-2331</link>
		<dc:creator>Dennis Lusk</dc:creator>
		<pubDate>Fri, 25 Sep 2009 06:39:06 +0000</pubDate>
		<guid isPermaLink="false">http://sandbox.geneticmaize.com/?p=170#comment-2331</guid>
		<description>&lt;p&gt;&lt;Cheap grain-fed meat is the single biggest problem facing agriculture, medicine, and arguably the planet.&gt;&lt;/p&gt;&lt;p&gt;There is no question that this statement is true. Most of the world outside of the Americas, animal fat is just a flavoring and meat for special occasions. &lt;/p&gt;&lt;p&gt;I would add another that is, unfortunately, gaining acceptance and that is ethanol production. It motivates industrial farms to put ever more land under cultivation and convert it fuel for automobiles. Ethanol production requires 20 times more water than gasoline, uses much valuable phosphorus,  and produces an astonishing amount of CO2 and other waste. It simply is a technology that is not even close to being perfected. It certainly should not be subsidized by the government, especially on such a vast scale.  If you think of all those automobiles in traffic as being vast herds of land mammals you will see that it is, indeed, a really bad idea. Ethanol production is just more carnivorism. It is also raising the price of food and causing starvation in Latin America and Africa. If Cap and Trade passes it will here too.&lt;/p&gt;</description>
		<content:encoded><![CDATA[<p>&lt;Cheap grain-fed meat is the single biggest problem facing agriculture, medicine, and arguably the planet.&gt;</p>
<p>There is no question that this statement is true. Most of the world outside of the Americas, animal fat is just a flavoring and meat for special occasions. </p>
<p>I would add another that is, unfortunately, gaining acceptance and that is ethanol production. It motivates industrial farms to put ever more land under cultivation and convert it fuel for automobiles. Ethanol production requires 20 times more water than gasoline, uses much valuable phosphorus,  and produces an astonishing amount of CO2 and other waste. It simply is a technology that is not even close to being perfected. It certainly should not be subsidized by the government, especially on such a vast scale.  If you think of all those automobiles in traffic as being vast herds of land mammals you will see that it is, indeed, a really bad idea. Ethanol production is just more carnivorism. It is also raising the price of food and causing starvation in Latin America and Africa. If Cap and Trade passes it will here too.</p>
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		<title>By: Karl Haro von Mogel</title>
		<link>http://www.biofortified.org/2009/08/eat-food-not-too-much-mostly-plants/#comment-2330</link>
		<dc:creator>Karl Haro von Mogel</dc:creator>
		<pubDate>Thu, 03 Sep 2009 11:05:04 +0000</pubDate>
		<guid isPermaLink="false">http://sandbox.geneticmaize.com/?p=170#comment-2330</guid>
		<description>&lt;p&gt;Sure, you can transform a plant with a gene gun, aka biolistics, the wikipedia article on it should be a good place to go first:&lt;br/&gt;http://en.wikipedia.org/wiki/Gene_gun&lt;/p&gt;&lt;p&gt;It works by shooting the gene into the cells, some of which arrive in the nucleus, and some of those get hooked up in the cell&#039;s chromosomes.&lt;/p&gt;&lt;p&gt;There is also another bacterium similar to agrobacterium that someone has found that can perform the same function, but I forget the name.&lt;/p&gt;</description>
		<content:encoded><![CDATA[<p>Sure, you can transform a plant with a gene gun, aka biolistics, the wikipedia article on it should be a good place to go first:<br /><a href="http://en.wikipedia.org/wiki/Gene_gun" rel="nofollow">http://en.wikipedia.org/wiki/Gene_gun</a></p>
<p>It works by shooting the gene into the cells, some of which arrive in the nucleus, and some of those get hooked up in the cell&#8217;s chromosomes.</p>
<p>There is also another bacterium similar to agrobacterium that someone has found that can perform the same function, but I forget the name.</p>
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		<title>By: sergeyadept</title>
		<link>http://www.biofortified.org/2009/08/eat-food-not-too-much-mostly-plants/#comment-2329</link>
		<dc:creator>sergeyadept</dc:creator>
		<pubDate>Thu, 03 Sep 2009 00:15:31 +0000</pubDate>
		<guid isPermaLink="false">http://sandbox.geneticmaize.com/?p=170#comment-2329</guid>
		<description>&lt;p&gt;Yes, nice &quot;pictoral step by step&quot;.  Thank you. &lt;br/&gt;And I am reading now &lt;a href=&quot;http://www.sciencedirect.com/science?_ob=ArticleURL&amp;_udi=B6VRV-4J6249R-1&amp;_user=10&amp;_rdoc=1&amp;_fmt=&amp;_orig=search&amp;_sort=d&amp;_docanchor=&amp;view=c&amp;_searchStrId=998562995&amp;_rerunOrigin=scholar.google&amp;_acct=C000050221&amp;_version=1&amp;_urlVersion=0&amp;_userid=10&amp;md5=b76535fb109af8c22139436dd6b09365&quot; rel=&quot;nofollow&quot;&gt;  this &lt;/a&gt; article.&lt;/p&gt;&lt;p&gt;But I can&#039;t find something about biolistics:(&lt;br/&gt;___________&lt;br/&gt;To Karl: Escherichia coli is a one species, isn&#039;t it. But let set aside E.coli. Can we transform plant without any help from Agrobacterium? I mean ANY help.&lt;/p&gt;</description>
		<content:encoded><![CDATA[<p>Yes, nice &quot;pictoral step by step&quot;.  Thank you. <br />And I am reading now <a href="http://www.sciencedirect.com/science?_ob=ArticleURL&amp;_udi=B6VRV-4J6249R-1&amp;_user=10&amp;_rdoc=1&amp;_fmt=&amp;_orig=search&amp;_sort=d&amp;_docanchor=&amp;view=c&amp;_searchStrId=998562995&amp;_rerunOrigin=scholar.google&amp;_acct=C000050221&amp;_version=1&amp;_urlVersion=0&amp;_userid=10&amp;md5=b76535fb109af8c22139436dd6b09365" rel="nofollow">  this </a> article.</p>
<p>But I can&#8217;t find something about biolistics:(<br />___________<br />To Karl: Escherichia coli is a one species, isn&#8217;t it. But let set aside E.coli. Can we transform plant without any help from Agrobacterium? I mean ANY help.</p>
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		<title>By: Karl Haro von Mogel</title>
		<link>http://www.biofortified.org/2009/08/eat-food-not-too-much-mostly-plants/#comment-2328</link>
		<dc:creator>Karl Haro von Mogel</dc:creator>
		<pubDate>Tue, 01 Sep 2009 11:08:10 +0000</pubDate>
		<guid isPermaLink="false">http://sandbox.geneticmaize.com/?p=170#comment-2328</guid>
		<description>&lt;p&gt;But I love the smell of yeast extract... mmmm...&lt;/p&gt;</description>
		<content:encoded><![CDATA[<p>But I love the smell of yeast extract&#8230; mmmm&#8230;</p>
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		<title>By: Anastasia</title>
		<link>http://www.biofortified.org/2009/08/eat-food-not-too-much-mostly-plants/#comment-2327</link>
		<dc:creator>Anastasia</dc:creator>
		<pubDate>Sun, 30 Aug 2009 10:22:04 +0000</pubDate>
		<guid isPermaLink="false">http://sandbox.geneticmaize.com/?p=170#comment-2327</guid>
		<description>&lt;p&gt;My biggest problem with using &lt;i&gt;E. coli&lt;/i&gt; as a lab rat is the stink. I hate the smell of bacterial cultures. Blarg.&lt;/p&gt;</description>
		<content:encoded><![CDATA[<p>My biggest problem with using <i>E. coli</i> as a lab rat is the stink. I hate the smell of bacterial cultures. Blarg.</p>
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		<title>By: Karl Haro von Mogel</title>
		<link>http://www.biofortified.org/2009/08/eat-food-not-too-much-mostly-plants/#comment-2326</link>
		<dc:creator>Karl Haro von Mogel</dc:creator>
		<pubDate>Sat, 29 Aug 2009 23:59:49 +0000</pubDate>
		<guid isPermaLink="false">http://sandbox.geneticmaize.com/?p=170#comment-2326</guid>
		<description>&lt;p&gt;I have heard other objections to the use of bacteria in genetic engineering. Thanks to Deborah &quot;They&#039;re putting bacteria in our food&quot; Koons Garcia, it is viewed as a scary practice. Especially when people learn that the common bacteria that is used to store genes and sequences in the long-term is E. coli. Oh noes!&lt;/p&gt;&lt;p&gt;The kind of E. coli that gives people diarrhea and the kind of E. coli used in labs are two different animals, err, prokaryotes. The former is a virulent pathogen while the latter is a tame, domesticated cell with its virulence removed. It has become the lab-rat of molecular biology, able to make copies of circular pieces of DNA containing your gene, indefinitely, until you need to use it. Usually, genetic engineers will make their genetic construct in an E. coli bacterium, and then transfer it to Agrobacterium to then transfer it into the plant.&lt;/p&gt;&lt;p&gt;There is always the small possibility that a piece of bacterial DNA could get into the plant, which is why you have to prove to regulators that only your DNA of interest is inserted into the plant and nothing else. Even if something slipped through, the chance of it causing any harm is exceedingly small - DNA doesn&#039;t itself make E. coli dangerous, it is how all of the DNA of the virulent pathogen together tells it how to infect our bodies. Given how difficult it can be to get the gene you want to insert into the plant and work properly, the chance of an intact, functional, dangerous gene from getting into the plant from the bacterium, and actually working in a completely different genetic environment is astronomically small. But it sure &lt;em&gt;sounds&lt;/em&gt; scary.&lt;/p&gt;</description>
		<content:encoded><![CDATA[<p>I have heard other objections to the use of bacteria in genetic engineering. Thanks to Deborah &quot;They&#8217;re putting bacteria in our food&quot; Koons Garcia, it is viewed as a scary practice. Especially when people learn that the common bacteria that is used to store genes and sequences in the long-term is E. coli. Oh noes!</p>
<p>The kind of E. coli that gives people diarrhea and the kind of E. coli used in labs are two different animals, err, prokaryotes. The former is a virulent pathogen while the latter is a tame, domesticated cell with its virulence removed. It has become the lab-rat of molecular biology, able to make copies of circular pieces of DNA containing your gene, indefinitely, until you need to use it. Usually, genetic engineers will make their genetic construct in an E. coli bacterium, and then transfer it to Agrobacterium to then transfer it into the plant.</p>
<p>There is always the small possibility that a piece of bacterial DNA could get into the plant, which is why you have to prove to regulators that only your DNA of interest is inserted into the plant and nothing else. Even if something slipped through, the chance of it causing any harm is exceedingly small &#8211; DNA doesn&#8217;t itself make E. coli dangerous, it is how all of the DNA of the virulent pathogen together tells it how to infect our bodies. Given how difficult it can be to get the gene you want to insert into the plant and work properly, the chance of an intact, functional, dangerous gene from getting into the plant from the bacterium, and actually working in a completely different genetic environment is astronomically small. But it sure <em>sounds</em> scary.</p>
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		<title>By: Anastasia</title>
		<link>http://www.biofortified.org/2009/08/eat-food-not-too-much-mostly-plants/#comment-2325</link>
		<dc:creator>Anastasia</dc:creator>
		<pubDate>Fri, 28 Aug 2009 14:25:32 +0000</pubDate>
		<guid isPermaLink="false">http://sandbox.geneticmaize.com/?p=170#comment-2325</guid>
		<description>&lt;p&gt;Hm. Actually, I&#039;m really sorry to say I don&#039;t know of a single book or article that does a really good job of describing the two methods. That doesn&#039;t mean there aren&#039;t lots of good articles that explain one or the other. &lt;/p&gt;&lt;p&gt;I like this  &lt;a href=&quot;http://ppge.ucdavis.edu/Transformation/transform1.cfm?14,14&quot; rel=&quot;nofollow&quot;&gt;pictoral step by step&lt;/a&gt; from UC Davis about Agrobacterium a lot, but it doesn&#039;t talk about things at the molecular level. &lt;/p&gt;&lt;p&gt;I&#039;ll keep an eye out for a book. That would be helpful!&lt;/p&gt;</description>
		<content:encoded><![CDATA[<p>Hm. Actually, I&#8217;m really sorry to say I don&#8217;t know of a single book or article that does a really good job of describing the two methods. That doesn&#8217;t mean there aren&#8217;t lots of good articles that explain one or the other. </p>
<p>I like this  <a href="http://ppge.ucdavis.edu/Transformation/transform1.cfm?14,14" rel="nofollow">pictoral step by step</a> from UC Davis about Agrobacterium a lot, but it doesn&#8217;t talk about things at the molecular level. </p>
<p>I&#8217;ll keep an eye out for a book. That would be helpful!</p>
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		<title>By: sergeyadept</title>
		<link>http://www.biofortified.org/2009/08/eat-food-not-too-much-mostly-plants/#comment-2324</link>
		<dc:creator>sergeyadept</dc:creator>
		<pubDate>Fri, 28 Aug 2009 05:59:09 +0000</pubDate>
		<guid isPermaLink="false">http://sandbox.geneticmaize.com/?p=170#comment-2324</guid>
		<description>&lt;p&gt;Thank you for your answer!:)&lt;br/&gt;Can you recommend some book or article, with information about linear DNA and biolistics? I can&#039;t got the idea of how this gene put himself in  plant genome.&lt;/p&gt;</description>
		<content:encoded><![CDATA[<p>Thank you for your answer!:)<br />Can you recommend some book or article, with information about linear DNA and biolistics? I can&#8217;t got the idea of how this gene put himself in  plant genome.</p>
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